Fibroblast Cells: A Sensing Bioelement for Glucose Detection by Impedance Spectroscopy

Abstract

Modifying the electrical properties of fibroblasts against various glucose concentrations can serve as a basis for a new, original sensing device. The aim of the present study is to test a new biosensor based on impedancemetry measurement using eukaryote cells. Fibroblast cells were grown on a small optically transparent indium tin oxide semiconductor electrode. Electrochemical impedance spectroscopy (EIS) was used to measure the effect of D-glucose on the electrical properties of fibroblast cells. Further analyses of the EIS results were performed using equivalent circuits in order to model the electrical flow through the interface. The linear calibration curve was established in the range 0-14 mM. The specification of the biosensors was verified using cytochalasin B as an inhibitor agent of the glucose transporters. The nonreactivity to sugars other than glucose was demonstrated. Such a biosensor could be applied to a more fundamental study of cell metabolism.