1H and 13C HR‐MAS spectroscopy of intact biopsy samples ex vivo and in vivo1H MRS study of human high grade gliomas

Abstract

High‐resolution magic angle spinning (HR‐MAS) one‐ and two‐dimensional ¹H and ¹³C nuclear magnetic resonance (NMR) spectroscopy has been used to study intact glioblastoma (GBM) brain tumour tissue. The results were compared with in vitro chemical extract and in vivo spectra. The resolution of ¹H one‐dimensional, ¹H TOCSY and ¹³C HSQC HR‐MAS spectra is comparable to that obtained on perchloric extracts. ¹³C HSQC HR‐MAS spectra have been particularly useful for the identification of 37 different metabolites in intact biopsy tumours, excluding water and DSS components. To our knowledge, this is the most detailed assignment of biochemical compounds obtained in intact human tissue, in particular in brain tumour tissue. Tissue degradation during the recording of the NMR experiment was avoided by keeping the sample at a temperature of 4°C. Detailed metabolical compositions of 10 GBM (six primary, two secondary and two unclassified) were obtained. A good correlation between ex vivo and in vivo MRS has been found. Copyright © 2004 John Wiley & Sons, Ltd.