High‐resolution magic angle spinning MRS of breast cancer tissue
Top Cited Papers
- 1 August 2002
- journal article
- research article
- Published by Wiley in NMR in Biomedicine
- Vol. 15 (5), 327-337
- https://doi.org/10.1002/nbm.775
Abstract
High‐resolution magic angle spinning (HR MAS) may develop into a new diagnostic tool for studying intact tissue samples, and several types of cancer have been investigated with promising results. In this study HR MAS spectra of breast cancer tissue from 10 patients have been compared to conventional high‐resolution spectra of perchloric acid extracts of the same tissue type. The HR MAS spectra show resolution comparable to spectra of extracts, and two‐dimensional techniques lead to identification of a majority of the constituents. More than 30 different metabolites have been detected and assigned. To our knowledge this is the most detailed assignment of biochemical components in intact human breast tissue. The spectra of intact breast cancer tissue differ from perchloric acid extracts by the presence of lipids and fewer signals in the low field region. HR MAS analysis of intact breast tissue specimens is a rapid method, providing spectra with resolution where relative quantification of the majority of the detected metabolites is possible. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
Funding Information
- The Norwegian Research Council (133673/420)
This publication has 35 references indexed in Scilit:
- Diagnosis of cancer in humans by 1H NMR of tissue biopsiesBiochemistry and Cell Biology, 1998
- Metabolite profiling by one- and two-dimensional NMR analysis of complex mixturesProgress in Nuclear Magnetic Resonance Spectroscopy, 1996
- Nuclear magnetic resonance spectroscopyAnalytical Chemistry, 1995
- Internal temperature calibration for 1H NMR spectroscopy studies of blood plasma and other biofluidsNMR in Biomedicine, 1994
- 1H NMR spectroscopic characterization of perchloric acid extracts from breast carcinomas and non‐involved breast tissueNMR in Biomedicine, 1994
- Differentiation of human prostate cancer from benign hypertrophy byin vitro1H NMRMagnetic Resonance in Medicine, 1992
- High‐Resolution 1H NMR spectroscopy studies of extracts of human cerebral neoplasmsMagnetic Resonance in Medicine, 1992
- Breast cancer treatment and natural history: new insights from results of screeningThe Lancet, 1992
- Contrast-Enhanced Magnetic Resonance Imaging of the BreastActa Oncologica, 1992
- Assignment of resonances in the 1H spectrum of rat brain by two‐dimensional shift correlated and j‐resolved NMR spectroscopyMagnetic Resonance in Medicine, 1991