Assessing microstructures of the cornea with Gabor-domain optical coherence microscopy: pathway for corneal physiology and diseases
- 13 March 2015
- journal article
- Published by Optica Publishing Group in Optics Letters
- Vol. 40 (6), 1113-1116
- https://doi.org/10.1364/ol.40.001113
Abstract
Gabor-domain optical coherence microscopy (GD-OCM) was applied ex vivo in the investigation of corneal cells and their surrounding microstructures with particular attention to the corneal endothelium. Experiments using fresh pig eyeballs, excised human corneal buttons from patients with Fuchs’ endothelial dystrophy (FED), and healthy donor corneas were conducted. Results show in a large field of view () high definition images of the different cell types and their surrounding microstructures through the full corneal thickness at both the central and peripheral locations of porcine corneas. Particularly, an image of the endothelial cells lining the bottom of the cornea is highlighted. As compared to healthy human corneas, the corneas of individuals with FED show characteristic microstructural alterations of the Descemet’s membrane and increased size and number of keratocytes. The GD-OCM-based imaging system developed may constitute a novel tool for corneal imaging and disease diagnosis. Also, importantly, it may provide insights into the mechanism of corneal physiology and pathology, particularly in diseases of the corneal endothelium.
Funding Information
- National Eye Institute (NEI) (EY007125)
- National Science Foundation (NSF) (IIP ENG / 1346453)
- New York State Foundation for Science, Technology and Innovation (NYSTAR) (C050070)
This publication has 26 references indexed in Scilit:
- Optical coherence tomography today: speed, contrast, and multimodalityJournal of Biomedical Optics, 2014
- Parallelized multi–graphics processing unit framework for high-speed Gabor-domain optical coherence microscopyJournal of Biomedical Optics, 2014
- Non-contact laser-scanning confocal microscopy of the human cornea in vivoContact Lens and Anterior Eye, 2014
- Cellular resolution optical coherence microscopy with high acquisition speed for in-vivo human skin volumetric imagingOptics Letters, 2011
- Donor and Recipient Endothelial Cell Population of the Transplanted Human Cornea: A Two-Dimensional Imaging StudyInvestigative Ophthalmology & Visual Science, 2010
- Confocal Microscopy in OphthalmologyAmerican Journal of Ophthalmology, 2009
- Ocular Tissue Imaging Using Ultrahigh-Resolution, Full-Field Optical Coherence TomographyInvestigative Ophthalmology & Visual Science, 2004
- Specular microscopic study of X-ray-irradiated rabbit corneaEye, 2004
- Specular microscopy of the corneal endothelium.British Journal of Ophthalmology, 1978
- The transparency of the mammalian corneaJournal Of Physiology-London, 1970