Cellular resolution optical coherence microscopy with high acquisition speed for in-vivo human skin volumetric imaging

Abstract

In this Letter, we report for the first time (to our knowledge) in-vivo volumetric optical coherence microscopy images of skin epidermal cells. We achieved micrometer-class resolution, $2\mathrm{\mu m}$ laterally and axially, with an acquisition speed of $23\mathrm{K}$ A-scans/s and over $90\mathrm{dB}$ sensitivity to a depth of $1\mathrm{mm}$ by employing a custom, liquid-lens-based, dynamic-focusing objective, a broadband light source, and a custom, astigmatism-corrected Czerny–Turner spectrometer with a high-speed complementary metal-oxide-semiconductor camera.