Comparative Analysis of the Genetic Structure and Chromosomal Location of the MurineMyD118(GADD45β) Gene

Abstract

The MyD118 (Gadd45β) protein is a member of a family of structurally related proteins, including Gadd45 (Gadd45α) and CR6 (Gadd45γ), that have critical roles in regulating growth arrest and apoptosis. The MyD118 and other members of its family display distinct patterns of expression in response to stimuli that induce differentiation, growth arrest, or apoptosis. Species-blot analysis showed that MyD118 is an evolutionarily conserved gene, and comparative sequence analysis showed that MyD118 has a gene structure similar to that of other members of its gene family. Comparison of putative transcription factor-binding sites found in sequences of this gene family provides evidence that p53 is involved in regulating the expression of MyD118 and that NF-κB may play a role in differential expression of MyD118 and Gadd45(Gadd45α). Fluorescence in situ hybridization localized the MyD118 gene to mouse chromosome band 10B5.3, correcting a previous assignment to mouse chromosome 9.