Rapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route
Open Access
- 10 April 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (7), 1691-1706
- https://doi.org/10.1093/nar/9.7.1691
Abstract
Efficient mechanised synthesis of heptadecadeoxyribonucleotides has been achieved on an economically small scale by an improved solid phase phosphotriester method on a polydimethylacrylamide resin. Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.Keywords
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