Discrimination of Mumps Virus Small Hydrophobic Gene Deletion Effects from Gene Translation Effects on Virus Virulence
- 15 June 2011
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 85 (12), 6082-6085
- https://doi.org/10.1128/jvi.02686-10
Abstract
Deletion of the small hydrophobic (SH) protein of certain paramyxoviruses has been found to result in attenuation, suggesting that the SH protein is a virulence factor. To investigate the role of the mumps virus (MuV) SH protein in virulence, multiple stop codons were introduced into the open reading frame (ORF) of a MuV molecular clone (r88-1961 SHstop ), preserving genome structure but precluding production of the SH protein. No differences in neurovirulence were seen between the wild-type and the SH stop viruses. In contrast, upon deletion of the SH gene, significant neuroattenuation was observed. These data indicate that the MuV SH protein is not a neurovirulence factor and highlight the importance of distinguishing gene deletion effects from protein-specific effects.Keywords
This publication has 29 references indexed in Scilit:
- Function of the Small Hydrophobic Protein of J ParamyxovirusJournal of Virology, 2011
- Function of the Respiratory Syncytial Virus Small Hydrophobic ProteinJournal of Virology, 2007
- Infection of Nonhuman Primates with Recombinant Human Metapneumovirus Lacking the SH, G, or M2-2 Protein Categorizes Each as a Nonessential Accessory Protein and Identifies Vaccine CandidatesJournal of Virology, 2005
- The small hydrophobic (SH) protein accumulates within lipid-raft structures of the Golgi complex during respiratory syncytial virus infectionJournal of General Virology, 2004
- The SH Integral Membrane Protein of the Paramyxovirus Simian Virus 5 Is Required To Block Apoptosis in MDBK CellsJournal of Virology, 2001
- Membrane Permeability Changes Induced inEscherichia coliby the SH Protein of Human Respiratory Syncytial VirusVirology, 1997
- Molecular characterization of mumps virus strains circulating during an epidemic in Eastern Switzerland 1992/93Archiv für die gesamte Virusforschung, 1994
- Efficient selection for high-expression transfectants with a novel eukaryotic vectorGene, 1991
- Virus and host cell-dependent variation in transcription of the mumps virus genomeJournal of General Virology, 1990
- Nucleotide sequence of the matrix, fusion and putative SH protein genes of mumps virus and their deduced amino acid sequencesVirus Research, 1989