Arabidopsis VASCULAR-RELATED NAC-DOMAIN6 Directly Regulates the Genes That Govern Programmed Cell Death and Secondary Wall Formation during Xylem Differentiation
- 1 October 2010
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 22 (10), 3461-3473
- https://doi.org/10.1105/tpc.110.075036
Abstract
Xylem consists of three types of cells: tracheary elements (TEs), parenchyma cells, and fiber cells. TE differentiation includes two essential processes, programmed cell death (PCD) and secondary cell wall formation. These two processes are tightly coupled. However, little is known about the molecular mechanisms underlying these processes. Here, we show that VASCULAR-RELATED NAC-DOMAIN6 (VND6), a master regulator of TEs, regulates some of the downstream genes involved in these processes in a coordinated manner. We first identified genes that are expressed downstream of VND6 but not downstream of SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN1 (SND1), a master regulator of xylem fiber cells, using transformed suspension culture cells in microarray experiments. We found that VND6 and SND1 governed distinct aspects of xylem formation, whereas they regulated a number of genes in common, specifically those related to secondary cell wall formation. Genes involved in TE-specific PCD were upregulated only by VND6. Moreover, we revealed that VND6 directly regulated genes that harbor a TE-specific cis-element, TERE, in their promoters. Thus, we found that VND6 is a direct regulator of genes related to PCD as well as to secondary wall formation.Keywords
This publication has 39 references indexed in Scilit:
- MYB83 Is a Direct Target of SND1 and Acts Redundantly with MYB46 in the Regulation of Secondary Cell Wall Biosynthesis in ArabidopsisPlant and Cell Physiology, 2009
- MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in ArabidopsisPlant Cell, 2009
- A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in ArabidopsisPlant Cell, 2008
- Cysteine proteases XCP1 and XCP2 aid micro‐autolysis within the intact central vacuole during xylogenesis in Arabidopsis rootsThe Plant Journal, 2008
- The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in ArabidopsisPlant Cell, 2007
- Participation of the Arabidopsis bHLH Factor GL3 in Trichome Initiation Regulatory EventsPlant Physiology, 2007
- Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformationJournal of Bioscience and Bioengineering, 2007
- NAC Transcription Factors, NST1 and NST3, Are Key Regulators of the Formation of Secondary Walls in Woody Tissues ofArabidopsisPlant Cell, 2007
- SND1, a NAC Domain Transcription Factor, Is a Key Regulator of Secondary Wall Synthesis in Fibers ofArabidopsisPlant Cell, 2006
- Statistical significance for genomewide studiesProceedings of the National Academy of Sciences of the United States of America, 2003