The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
Top Cited Papers
- 21 September 2007
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 19 (9), 2776-2792
- https://doi.org/10.1105/tpc.107.053678
Abstract
We demonstrate that the Arabidopsis thaliana MYB46 transcription factor is a direct target of SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN1 (SND1), which is a key transcriptional activator regulating the developmental program of secondary wall biosynthesis. The MYB46 gene is expressed predominantly in fibers and vessels in stems, and its encoded protein is targeted to the nucleus and can activate transcription. MYB46 gene expression was shown to be regulated by SND1, and transactivation analysis demonstrated that SND1 as well as its close homologs were able to activate the MYB46 promoter. Electrophoretic mobility shift assays and chromatin immunoprecipitation experiments revealed that SND1 binds to the MYB46 promoter. Dominant repression of MYB46 caused a drastic reduction in the secondary wall thickening of fibers and vessels. Overexpression of MYB46 resulted in an activation of the biosynthetic pathways of cellulose, xylan, and lignin and concomitantly led to ectopic deposition of secondary walls in cells that are normally nonsclerenchymatous. In addition, the expression of two secondary wall-associated transcription factors, MYB85 and KNAT7, was highly upregulated by MYB46 overexpression. These results demonstrate that MYB46 is a direct target of SND1 and is another key player in the transcriptional network involved in the regulation of secondary wall biosynthesis in Arabidopsis.Keywords
This publication has 29 references indexed in Scilit:
- Arabidopsis irregular xylem8andirregular xylem9: Implications for the Complexity of Glucuronoxylan BiosynthesisPlant Cell, 2007
- NAC Transcription Factors, NST1 and NST3, Are Key Regulators of the Formation of Secondary Walls in Woody Tissues ofArabidopsisPlant Cell, 2007
- EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesisThe Plant Journal, 2005
- Identification of the minimal repression domain of SUPERMAN shows that the DLELRL hexapeptide is both necessary and sufficient for repression of transcription in ArabidopsisBiochemical and Biophysical Research Communications, 2004
- Lignin BiosynthesisAnnual Review of Plant Biology, 2003
- Signal transduction in maize and Arabidopsis mesophyll protoplasts.2001
- The R2R3-MYB gene family in Arabidopsis thalianaCurrent Opinion in Plant Biology, 2001
- The Arabidopsis ATHB-8 HD-Zip Protein Acts as a Differentiation-Promoting Transcription Factor of the Vascular MeristemsPlant Physiology, 2001
- Functional Interactions of Lanthanum and Phospholipase D with the Abscisic Acid Signaling Effectors VP1 and ABI1-1 in Rice ProtoplastsJournal of Biological Chemistry, 2001
- The AmMYB308 and AmMYB330 Transcription Factors from Antirrhinum Regulate Phenylpropanoid and Lignin Biosynthesis in Transgenic TobaccoPlant Cell, 1998