Enzyme clustering accelerates processing of intermediates through metabolic channeling
Open Access
- 28 September 2014
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 32 (10), 1011-1018
- https://doi.org/10.1038/nbt.3018
Abstract
An elegant mathematical model supported by experiments in Escherichia coli demonstrates how clustering enzymes can efficiently channel intermediates from one enzyme to the next, facilitating rational engineering of metabolism. We present a quantitative model to demonstrate that coclustering multiple enzymes into compact agglomerates accelerates the processing of intermediates, yielding the same efficiency benefits as direct channeling, a well-known mechanism in which enzymes are funneled between enzyme active sites through a physical tunnel. The model predicts the separation and size of coclusters that maximize metabolic efficiency, and this prediction is in agreement with previously reported spacings between coclusters in mammalian cells. For direct validation, we study a metabolic branch point in Escherichia coli and experimentally confirm the model prediction that enzyme agglomerates can accelerate the processing of a shared intermediate by one branch, and thus regulate steady-state flux division. Our studies establish a quantitative framework to understand coclustering-mediated metabolic channeling and its application to both efficiency improvement and metabolic regulation.Keywords
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