Microtubule-assisted mechanism for functional metabolic macromolecular complex formation
- 6 July 2010
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (29), 12872-12876
- https://doi.org/10.1073/pnas.1008451107
Abstract
Evidence has been presented for a metabolic multienzyme complex, the purinosome, that participates in de novo purine biosynthesis to form clusters in the cytoplasm of living cells under purine-depleted conditions. Here we identified, using fluorescent live cell imaging, that a microtubule network appears to physically control the spatial distribution of purinosomes in the cytoplasm. Application of a cell-based assay measuring the rate of de novo purine biosynthesis confirmed that the metabolic activity of purinosomes was significantly suppressed in the absence of microtubules. Collectively, we propose a microtubule-assisted mechanism for functional purinosome formation in HeLa cells.Keywords
This publication has 12 references indexed in Scilit:
- Dynamic Regulation of a Metabolic Multi-enzyme Complex by Protein Kinase CK2*Journal of Biological Chemistry, 2010
- Reversible Compartmentalization of de Novo Purine Biosynthetic Complexes in Living CellsScience, 2008
- Feedback Inhibition of Amidophosphoribosyltransferase Regulates the Rate of Cell Growth via Purine Nucleotide, DNA, and Protein SynthesesPublished by Elsevier BV ,2001
- A potential role of the cytoskeleton ofSaccharomyces cerevisiae in a functional organization of glycolytic enzymesYeast, 1999
- Cell Cycle-Related Changes in F-Actin Distribution Are Correlated with Glycolytic ActivityExperimental Cell Research, 1995
- Microcompartmentation of glycolytic enzymes in cultured cells.1992
- Aldolase exists in both the fluid and solid phases of cytoplasm [published erratum appears in J Cell Biol 1988 Dec;107(6 Pt 1):following 2463]The Journal of cell biology, 1988
- COMPLEXES OF SEQUENTIAL METABOLIC ENZYMESAnnual Review of Biochemistry, 1987
- Decreased purine synthesis during amino acid starvation of human lymphoblasts.Journal of Biological Chemistry, 1982
- Characterization of the enzyme complex involving the folate-requiring enzymes of de novo purine biosynthesisBiochemistry, 1980