Observation of single‐stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy
- 18 September 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (24), 5671-5675
- https://doi.org/10.1016/j.febslet.2006.09.017
Abstract
Atomic force microscopy was used to image single-stranded DNA (ssDNA) adsorbed on mica modified by Mg(2+), by 3-aminopropyltriethoxysilane or on modified highly oriented pyrolytic graphite (HOPG). ssDNA molecules on mica have compact structures with lumps, loops and super twisting, while on modified HOPG graphite ssDNA molecules adopt a conformation without secondary structures. We have shown that the immobilization of ssDNA under standard conditions on modified HOPG eliminates intramolecular base-pairing, thus this method could be important for studying certain processes involving ssDNA in more details.This publication has 19 references indexed in Scilit:
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