SLC35F2, a Transporter Sporadically Mutated in the Untranslated Region, Promotes Growth, Migration, and Invasion of Bladder Cancer Cells
Open Access
- 5 January 2021
- Vol. 10 (1), 80
- https://doi.org/10.3390/cells10010080
Abstract
Bladder cancer is a very heterogeneous disease and the molecular mechanisms of carcinogenesis and progression are insufficiently investigated. From the DNA sequencing analysis of matched non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC) samples from eight patients, we identified the tumour-associated gene SLC35F2 to be mutated in the 5′ and 3′ untranslated region (UTR). One mutation in 3′UTR increased the luciferase activity reporter, suggesting its influence on the protein expression of SLC35F2. The mRNA level of SLC35F2 was increased in MIBC compared with NMIBC. Furthermore, in immunohistochemical staining, we observed a strong intensity of SLC35F2 in single tumour cells and in the border cells of solid tumour areas with an atypical accumulation around the nucleus, especially in the MIBC. This suggests that SLC35F2 might be highly expressed in aggressive and invasive tumour cells. Moreover, knockdown of SLC35F2 repressed the growth of bladder cancer cells in the monolayer and spheroid model and suppressed migration and invasion of bladder cancer cells. In conclusion, we suggest that SLC35F2 is involved in bladder cancer progression and might provide a new therapeutic approach, for example, by the anti-cancer drug YM155, a cargo of the SLC35F2 transporter.Keywords
This publication has 33 references indexed in Scilit:
- Muscle‐invasive bladder cancer: evaluating treatment and survival in the National Cancer Data BaseBJU International, 2014
- Influence on the behavior of lung cancer H1299 cells by silencing SLC35F2 expressionCancer Cell International, 2013
- Roles of the nucleotide sugar transporters (SLC35 family) in health and diseaseMolecular Aspects of Medicine, 2013
- X-ray, lensing and Sunyaev-Zel'dovich triaxial analysis of Abell 1835 out to R200Monthly Notices of the Royal Astronomical Society, 2012
- Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological stagingMolecular Medicine Reports, 2011
- Association Between Smoking and Risk of Bladder Cancer Among Men and WomenJAMA, 2011
- Broad spectrum and potent antitumor activities of YM155, a novel small‐molecule survivin suppressant, in a wide variety of human cancer cell lines and xenograft modelsCancer Science, 2011
- Analysis and update of the human solute carrier (SLC) gene superfamilyHuman Genomics, 2009
- SOME TUMORS OF THE BLADDER ARE POLYCLONAL IN ORIGINJournal of Urology, 2002
- Construction of a Transcription Map around the Gene for Ataxia Telangiectasia: Identification of at Least Four Novel GenesGenomics, 1997