Influence on the behavior of lung cancer H1299 cells by silencing SLC35F2 expression
Open Access
- 23 July 2013
- journal article
- Published by Springer Science and Business Media LLC in Cancer Cell International
- Vol. 13 (1), 73
- https://doi.org/10.1186/1475-2867-13-73
Abstract
Background To investigate the effects of RNA interference-mediated downregulation of Human Solute Carrier Family 35 member F2 (SLC35F2) expression on the biological behavior of lung cancer H1299 cells. Methods The lentiviral vector of small interfering RNA targeting SLC35F2 was introduced into H1299 cells by liposome-mediated transfection. Expression of the SLC35F2 protein was measured by western blot. The proliferation of H1299 cells was determined by Cell Counting Kit-8 assay. The migration of H1299 cells was measured by Transwell migration assay. Cell cycle analysis used fluorescence-activated cell sorting. Results SLC35F2 expression was markedly downregulated in H1299 cell clone (transfected with the lentiviral vector harboring small interfering RNA targeting SLC35F2). Proliferation decreased significantly compared with that of non-transfected H1299 cells. Transwell migration assay showed that fewer cells moved through the artificial basement membrane compared with untransfected H1299 cells (38.3 ± 5.7 vs. 113.5 ± 8.5, P < 0.05). The cell cycle of H1299 cells was changed, the percentage of H1299 cells in S and G2/M phases being significantly decreased compared with untransfected H1299 cells (S phase: 15.3% ± 3.0% vs. 27.0% ± 5.4%, P > 0.05; G2/M phase; 3.0% ± 1.1% vs. 10.5% ± 1.7%, P < 0.05), whereas the percentage of H1299 cells in G0/G1 phase increased markedly (81.7% ± 4.0% vs. 62.5% ± 1.9%, P < 0.05). Conclusion RNA interference-mediated downregulation of SLC35F2 expression by lentiviral vector can attenuate the proliferation, migration and invasion of H1299 cells.Keywords
This publication has 11 references indexed in Scilit:
- Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological stagingMolecular Medicine Reports, 2011
- Tissue-Specific mRNA Expression Profiles of Human Solute Carrier 35 TransportersDrug Metabolism and Pharmacokinetics, 2009
- The Epithelial Membrane Protein 1 is a Novel Tight Junction Protein of the Blood—Brain BarrierJournal of Cerebral Blood Flow & Metabolism, 2008
- Predicting 5‐fluorouracil chemosensitivity of liver metastases from colorectal cancer using primary tumor specimens: Three‐gene expression model predicts clinical responseInternational Journal of Cancer, 2006
- Molecular Cloning and Characterization of a Novel 3′-Phosphoadenosine 5′-Phosphosulfate Transporter, PAPST2Published by Elsevier BV ,2006
- Identification and characterization of human Golgi nucleotide sugar transporter SLC35D2, a novel member of the SLC35 nucleotide sugar transporter familyGenomics, 2005
- Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35)Pflügers Archiv - European Journal of Physiology, 2004
- Increased expression of UDP-galactose transporter messenger RNA in human colon cancer tissues and its implication in synthesis of Thomsen-Friedenreich antigen and sialyl Lewis A/X determinants.2001
- Molecular characterization of human UDP‐glucuronic acid/UDP‐N‐acetylgalactosamine transporter, a novel nucleotide sugar transporter with dual substrate specificityFEBS Letters, 2001
- Construction of a Transcription Map around the Gene for Ataxia Telangiectasia: Identification of at Least Four Novel GenesGenomics, 1997