Insect Odorant Receptors Are Molecular Targets of the Insect Repellent DEET
- 28 March 2008
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 319 (5871), 1838-1842
- https://doi.org/10.1126/science.1153121
Abstract
DEET ( N,N -diethyl-meta-toluamide) is the world's most widely used topical insect repellent, with broad effectiveness against most insects. Its mechanism of action and molecular target remain unknown. Here, we show that DEET blocks electrophysiological responses of olfactory sensory neurons to attractive odors in Anopheles gambiae and Drosophila melanogaster . DEET inhibits behavioral attraction to food odors in Drosophila , and this inhibition requires the highly conserved olfactory co-receptor OR83b. DEET inhibits odor-evoked currents mediated by the insect odorant receptor complex, comprising a ligand-binding subunit and OR83b. We conclude that DEET masks host odor by inhibiting subsets of heteromeric insect odorant receptors that require the OR83b co-receptor. The identification of candidate molecular targets for the action of DEET may aid in the design of safer and more effective insect repellents.This publication has 25 references indexed in Scilit:
- Membrane topology of the Drosophila OR83b odorant receptorFEBS Letters, 2007
- Odor Coding in the Maxillary Palp of the Malaria Vector Mosquito Anopheles gambiaeCurrent Biology, 2007
- A general model of G protein‐coupled receptor sequences and its application to detect remote homologsProtein Science, 2006
- Atypical Membrane Topology and Heteromeric Function of Drosophila Odorant Receptors In VivoPLoS Biology, 2006
- Chemosensory Coding by Neurons in the Coeloconic Sensilla of theDrosophilaAntennaJournal of Neuroscience, 2005
- Insect Sex-Pheromone Signals Mediated by Specific Combinations of Olfactory ReceptorsScience, 2005
- Repellency of deet and SS220 applied to skin involves olfactory sensing by two species of ticksMedical and Veterinary Entomology, 2005
- Or83b Encodes a Broadly Expressed Odorant Receptor Essential for Drosophila OlfactionNeuron, 2004
- The Molecular Basis of Odor Coding in the Drosophila AntennaCell, 2004
- ODOR-MEDIATED BEHAVIOR OF AFROTROPICAL MALARIA MOSQUITOESAnnual Review of Entomology, 1999