α-l-arabinofuranosidase production byAspergillus nidulans
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 115 (1), 107-112
- https://doi.org/10.1111/j.1574-6968.1994.tb06622.x
Abstract
The effects of some physico-chemical parameters on production of extracellular α-L-arabinofuranosidase by Aspergillus nidulans were examined. Highest levels of α-L-arabinofuranosidase were generated with cultures grown on 1% (w/v) purified beet pulp arabinan at 30°C and at an initial pH of 7.0. The enzyme was shown to be very sensitive to the action of proteases. Zymogram overlay of a protein profile obtained by SDS-PAGE revealed the occurrence of a band (Mr 36 000) exhibiting α-L-arabinofuranosidase activity. The isoelectric pH of the enzyme lay near 4.3. Temperature and pH optima for the activity of crude α-L-arabinofuranosidase preparations were 55°C and 5.5, respectively. Enzyme activity was greatly reduced by thiol reagents such as Hg2+ and p-hydroxymercuribenzoate and showed a Km value of 2.7 mM on p-nitrophenyl α-L-arabinofuranoside as substrate.Keywords
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