The cDNA of the two isoforms of bovine cGMP‐dependent protein kinase
- 17 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2), 191-196
- https://doi.org/10.1016/0014-5793(89)81453-x
Abstract
CDNAs encoding the isoform Iα of the cGMP‐dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in λgt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207–4218]. Alternate cDNA clones were isolated which code for a protein slightly different within the aminoterminal part from the known amino acid sequence. These alternate cDNAs contain the sequence of a peptide identified in the isoform 1β of cGMP‐dependent protein kinase. Northern blot analysis of poly(A)+ RNA from bovine trachea smooth muscle indicated the presence of two different mRNA species of about 6.2 kb.Keywords
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