Human Intestinal Mast Cells Are Capable of Producing Different Cytokine Profiles: Role of IgE Receptor Cross-Linking and IL-4
Open Access
- 1 January 2000
- journal article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 164 (1), 43-48
- https://doi.org/10.4049/jimmunol.164.1.43
Abstract
Mast cells are recognized as a new type of immunoregulatory cells capable of producing different cytokines. So far, little is known about the cytokine profile of mature human mast cells isolated from intestinal tissue and cultured in the presence of stem cell factor (SCF). We observed that these cells express the proinflammatory cytokines TNF-α, IL-1β, IL-6, IL-8, IL-16, and IL-18 without further stimulation. Both IgE-dependent and IgE-independent agonists (e.g., Gram-negative bacteria) enhanced expression of TNF-α. Another set of cytokines consisting of IL-3, IL-5, IL-9, and IL-13 was expressed following activation by IgE receptor cross-linking. If mast cells were cultured in the presence of IL-4 and SCF, the production and release of IL-3, IL-5, and IL-13 was increased up to 4-fold compared with mast cells cultured with SCF alone. By contrast, IL-6 expression was completely blocked in response to culture with IL-4. In summary, our data show that mature human mast cells produce proinflammatory cytokines that may be up-regulated following triggering with IgE-independent agonists such as bacteria, whereas activation by IgE receptor cross-linking results in the expression of Th2-type cytokines. IL-4 enhances the expression of Th2-type cytokines but does not affect or even down-regulates proinflammatory cytokines.Keywords
This publication has 22 references indexed in Scilit:
- Mast cells are an important cellular source of tumour necrosis factor α in human intestinal tissueGut, 1999
- Stem Cell Factor–Dependent Survival, Proliferation and Enhanced Releasability of Purified Mature Mast Cells Isolated from Human Intestinal TissueInternational Archives of Allergy and Immunology, 1999
- Functional diversity of helper T lymphocytesNature, 1996
- Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-αNature, 1996
- Critical protective role of mast cells in a model of acute septic peritonitisNature, 1996
- Constitutive and Inducible Cytokine mRNA Expression in the Human Mast Cell Line HMC-1Scandinavian Journal of Immunology, 1995
- Phenotypic Characterization of the Human Mast‐Cell Line HMC‐1Scandinavian Journal of Immunology, 1994
- Human peripheral blood basophils primed by interleukin 3 (IL-3) produce IL-4 in response to immunoglobulin E receptor stimulation.The Journal of Experimental Medicine, 1993
- Mast cells as a source of multifunctional cytokinesImmunology Today, 1990
- Interleukin 5 modifies histamine release and leukotriene generation by human basophils in response to diverse agonists.The Journal of Experimental Medicine, 1990