Polymorphisms within exon 9 but not intron 8 of the vitamin D receptor are associated with the nephropathic complication of type‐2 diabetes
- 19 August 2010
- journal article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 37 (6), 493-497
- https://doi.org/10.1111/j.1744-313x.2010.00953.x
Abstract
Background: The impact of several environmental and genetic factors on diabetes and its complications is well documented but there is an urgent need to understand more about genetic risk factors associated with this disease. The present study was aimed at examining the two single nucleotide polymorphisms (SNP) in intron 8 and exon 9 of the vitamin D receptor (VDR) gene in nephropathic and non-nephropathic type-2 diabetic patients. Material and methods: In this clinical study, peripheral blood samples were obtained from 100 type-2 diabetic patients, 100 nephropathic type-2 diabetic patients and 100 healthy controls. DNA was extracted and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to examine two SNP polymorphisms within the VDR gene. Results: Our results showed a significant difference in the Taq-1 evaluated genotypes of exon 9 within the VDR gene of diabetic individuals with (p=0.012) and without (p<0.001) nephropathy. Analysis of the Taq-1 evaluated alleles of nephropathic (p=0.917) and none-nephropathic (p=1.000) did not show a significant difference. We also evaluated the intron 8 Apa-1 alleles in patients with (p=0.480) and without nephropathy (p=0.543) and determined there were no differences between these groups. Our results also showed that the frequency of Apa-1 genotypes did not differ in nephropathic (p=0.224) and none-nephropathic (p=0.236) diabetic patients. Conclusion: Based on our results, it can be concluded that VDR and its functional polymorphism in exon 9 may play an important role in pathogenesis of type-2 diabetes and more investigations are required to clarify their role in nephropathy.Full TexKeywords
This publication has 26 references indexed in Scilit:
- The impact of vitamin D deficiency on diabetes and cardiovascular riskCurrent Opinion in Endocrinology, Diabetes and Obesity, 2010
- Nephropathic complication of type-2 diabetes is following pattern of autoimmune diseases?Diabetes Research and Clinical Practice, 2010
- Association of exon 9 but not intron 8 VDR polymorphisms with occult HBV infection in south‐eastern Iranian patientsJournal of Gastroenterology and Hepatology, 2009
- Is CCR5-Δ32 mutation associated with diabetic nephropathy in type 2 diabetes?Annals of Saudi Medicine, 2009
- Analyses of associations with asthma in four asthma population samples from Canada and AustraliaHuman Genetics, 2009
- Vitamin D receptor (FokI, BsmI and TaqI) gene polymorphisms and type 2 diabetes mellitus : A North Indian studyIndian Journal of Medical Sciences, 2009
- Glycine treatment decreases proinflammatory cytokines and increases interferon-γ in patients with Type 2 diabetesJournal of Endocrinological Investigation, 2008
- Iron Indices and Vitamin D Receptor Polymorphisms in Hemodialysis PatientsAdvances in Chronic Kidney Disease, 2008
- Family-based analysis of vitamin D receptor gene polymorphisms and type 1 diabetes in the population of South CroatiaJournal of Human Genetics, 2007
- Clinical risk factors and the role of VDR gene polymorphisms in diabetic retinopathy in Polish type 2 diabetes patientsActa Diabetologica, 2006