The breast cancer tumor suppressor BRCA2 promotes the specific targeting of RAD51 to single-stranded DNA
- 22 August 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Structural & Molecular Biology
- Vol. 17 (10), 1263-1265
- https://doi.org/10.1038/nsmb.1905
Abstract
Individuals with BRCA2 mutations are predisposed to breast cancers owing to genome instability. To determine the functions of BRCA 2, the human protein was purified. It was found to bind selectively to single-stranded DNA (ssDNA), and to ssDNA in tailed duplexes and replication fork structures. Monomeric and dimeric forms of BRCA 2 were observed by EM. BRCA 2 directed the binding of RA D51 recombinase to ssDNA, reduced the binding of RA D51 to duplex DNA and stimulated RA D51-mediated DNA strand exchange. These observations provide a molecular basis for the role of BRCA 2 in the maintenance of genome stability.Keywords
This publication has 21 references indexed in Scilit:
- Mitotic homologous recombination maintains genomic stability and suppresses tumorigenesisNature Reviews Molecular Cell Biology, 2010
- The BRC repeats of human BRCA2 differentially regulate RAD51 binding on single- versus double-stranded DNA to stimulate strand exchangeProceedings of the National Academy of Sciences of the United States of America, 2009
- The Carboxyl Terminus of Brca2 Links the Disassembly of Rad51 Complexes to Mitotic EntryCurrent Biology, 2009
- PALB2 is an integral component of the BRCA complex required for homologous recombination repairProceedings of the National Academy of Sciences of the United States of America, 2009
- The BRC Repeats of BRCA2 Modulate the DNA-Binding Selectivity of RAD51Cell, 2009
- Interactions between human BRCA2 protein and the meiosis-specific recombinase DMC1The EMBO Journal, 2007
- Dss1 Interaction with Brh2 as a Regulatory Mechanism for Recombinational RepairMolecular and Cellular Biology, 2007
- A region of human BRCA2 containing multiple BRC repeats promotes RAD51-mediated strand exchangeNucleic Acids Research, 2006
- Inactivation of RAD52 aggravates RAD54 defects in mice but not in Schizosaccharomyces pombeDNA Repair, 2005
- Functional Interactions among Yeast Rad51 Recombinase, Rad52 Mediator, and Replication Protein A in DNA Strand ExchangeOnline Journal of Public Health Informatics, 2000