Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real-Time PCR
- 1 April 2010
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 48 (4), 1105-1111
- https://doi.org/10.1128/jcm.01442-09
Abstract
Chronic infection with hepatitis B virus (HBV) is an important cause of cirrhosis and cancer of the liver. HBV is currently classified into eight genotypes, A to H. Accumulated evidence shows that the genotype influences both the clinical course of infection and the response to treatment. We describe a new method for genotyping based on TaqMan real-time PCR, which identifies all HBV genotypes without post-PCR processing. In this assay, each sample is processed in four multiplex real-time PCRs, each targeting two or three genotype-specific segments of HBV. By analyzing 185 samples representing all genotypes and different proportions of genotype mixtures, we could validate high accuracy of the assay. We conclude that this new assay represents a significant advancement for both diagnostics and clinical research because it is accurate, practical, and based on a technique that is well established in many virological laboratories.Keywords
This publication has 32 references indexed in Scilit:
- New Complex Recombinant Genotype of Hepatitis B Virus Identified in VietnamJournal of Virology, 2008
- Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real-Time PCR and Two-Step Melting Curve AnalysisJournal of Clinical Microbiology, 2006
- Increased hepatocarcinogenic potential of hepatitis B virus genotype A in Bantu‐speaking sub‐saharan AfricansJournal of Medical Virology, 2005
- Genetic Diversity of Hepatitis B Virus Strains Derived Worldwide: Genotypes, Subgenotypes, and HBsAg SubtypesIntervirology, 2004
- High prevalence of mixed genotype infections in hepatitis B virus infected intravenous drug usersJournal of Medical Virology, 2004
- Evaluation of the INNO-LiPA HBV Genotyping Assay for Determination of Hepatitis B Virus GenotypeJournal of Clinical Microbiology, 2003
- HBV genotype B is associated with better response to interferon therapy in HBeAg(+) chronic hepatitis than genotype CHepatology, 2002
- Clinical significance of hepatitis B virus genotypesHepatology, 2002
- Hepatitis B virus DNA levels, precore mutations, genotypes and histological activity in chronic hepatitis BJournal of Viral Hepatitis, 2000
- Genotypes, nt 1858 Variants, and Geographic Origin of Hepatitis B Virus—Large‐Scale Analysis Using a New Genotyping MethodThe Journal of Infectious Diseases, 1997