Synthesis and Application of CeO2/SnS2 Heterostructures as a Highly Efficient Coreaction Accelerator in the Luminol–Dissolved O2 System for Ultrasensitive Biomarkers Immunoassay
- 9 October 2019
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 91 (21), 14066-14073
- https://doi.org/10.1021/acs.analchem.9b03796
Abstract
Electrocheluminescence immunoassay amplified by coreaction accelerators has experienced major breakthroughs in ultrasensitive detection of biomarkers. Herein, CeO2/SnS2 heterostructures were synthesized and applied as novel coreaction accelerator to enhance the ECL efficiency of luminol-dissolved O2 system for the first time. Benefiting from the well-matched lattice spacing, ultrafine CeO2 nanoparticles (NPs) were in situ grew on layered SnS2 nanosheets (NSs) with improved dispersion. CeO2/SnS2 as a electroactive substrate can remarkably accelerate the generation of abundant superoxide anion radicals (O2•−) to react with luminol anion radical (L•−), achieving about 2-fold stronger ECL intensity than pure CeO2 NPs. To avoid harsh chemical synthesis of conventional ECL labels and simplify antibody conjugation process, ferritin (Ft) was served as a natural nanocarrier to immobilize luminol molecules ([email protected]) via one-step linkage, whose protein nanocage can easily connect with detection-antibody. Moreover, a robust site-oriented immobilization strategy using HWRGWVC heptapeptide as specific capturer was further adopted to maintain the bioactivity of capture-antibody on the amine-functionalized CeO2/SnS2 surface, which promoted the incubation efficiency markedly. On account of this advanced sensing strategy, a brand-new biosensor was constructed for the accurate detection of heart failure biomarkers, which performed favorable linearity in the range of 0.0001 - 50 ng/mL and achieved the detection limit of 36 fg/mL.Keywords
Funding Information
- Ministry of Science and Technology of the People's Republic of China (21627809)
- Shandong Province (ts201712052)
- National Natural Science Foundation of China (21505051, 21575050, 21777056)
- Jinan Scientific Research Leader Workshop Project (2018GXRC024)
This publication has 33 references indexed in Scilit:
- CuS as co-reaction accelerator in PTCA-K2S2O8 system for enhancing electrochemiluminescence behavior of PTCA and its application in detection of amyloid-β proteinBiosensors and Bioelectronics, 2019
- An ultratrace assay of arsenite based on the synergistic quenching effect of Ru(bpy)32+ and arsenite on the electrochemiluminescence of Au-g-C3N4 nanosheetsChemical Communications, 2018
- An ultrasensitive electrochemiluminescence biosensor for multiple detection of microRNAs based on a novel dual circuit catalyzed hairpin assemblyChemical Communications, 2018
- Silver Ions as Novel Coreaction Accelerator for Remarkably Enhanced Electrochemiluminescence in a PTCA–S2O82– System and Its Application in an Ultrasensitive Assay for Mercury IonsAnalytical Chemistry, 2018
- Electrochemiluminescence Peptide-Based Biosensor with Hetero-Nanostructures as Coreaction Accelerator for the Ultrasensitive Determination of TryptaseAnalytical Chemistry, 2018
- Ceria nanocrystals decorated mesoporous silica nanoparticle based ROS-scavenging tissue adhesive for highly efficient regenerative wound healingBiomaterials, 2018
- Ternary Electrochemiluminescence System Based on Rubrene Microrods as Luminophore and Pt Nanomaterials as Coreaction Accelerator for Ultrasensitive Detection of MicroRNA from Cancer CellsAnalytical Chemistry, 2017
- A novel metal–organic framework loaded with abundant N-(aminobutyl)-N-(ethylisoluminol) as a high-efficiency electrochemiluminescence indicator for sensitive detection of mucin1 on cancer cellsChemical Communications, 2017
- Visual electrochemiluminescence detection of telomerase activity based on multifunctional Au nanoparticles modified with G-quadruplex deoxyribozyme and luminolChemical Communications, 2014
- A novel electrochemiluminescence strategy for ultrasensitive DNA assay using luminol functionalized gold nanoparticles multi-labeling and amplification of gold nanoparticles and biotin–streptavidin systemChemical Communications, 2010