Electrochemiluminescence Peptide-Based Biosensor with Hetero-Nanostructures as Coreaction Accelerator for the Ultrasensitive Determination of Tryptase
- 16 January 2018
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 90 (3), 2263-2270
- https://doi.org/10.1021/acs.analchem.7b04631
Abstract
In this work, a luminol-centric biosensor was constructed for the ultrasensitive detection of tryptase (TPS) combining dissolved O2 as the endogenous coreactant and Au-Ag-Pt hetero-nanostructures (AAPHNs) as co-reaction accelerator. Dissolved O2 could rapidly generate superoxide anion radical (O2•−) with the catalysis of AAPHNs to in situ react with luminol anion radical (L•−) to generate excited-state species 3-aminophthalate (AP2−*) for emitting ECL signal, resulting in a remarkable “single on” state. In order to further improve the sensitivity of the sensor, we employed self-assembled DNA nanotubes (DNANTs) as a carrier to immobilize the luminophore of doxorubicin-luminol (Dox-Lu) complex. In this assay system, target tryptase could directly induce the cleavage of vasoactive intestinal peptide (VIP), which caused the ECL probe of DNANTs-Dox-Lu releasing from the electrode surface to obtain a significant "signal off" state. By changing the signal from "on" to "off", the proposed ECL peptide-based biosensor for TPS detection achieved a dynamic concentration range (2.5 pg/mL-200 ng/mL) with an extremely low detection limit of 0.81 pg/mL. This work presented a new signal amplification method for the construction of the sensor based on the luminol-dissolved O2 ECL system.Funding Information
- Ministry of Education of the People's Republic of China (XDJK2016E055)
- National Natural Science Foundation of China (21575116, 21675129, 21775124, 51473136)
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