High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease
- 1 May 2007
- Vol. 56 (5), 669-675
- https://doi.org/10.1136/gut.2006.099796
Abstract
Background: It is not clear which species of bacteria may be involved in inflammatory bowel disease (IBD). One way of determining which bacteria might be likely candidates is to use culture-independent methods to identify microorganisms that are present in diseased tissues but not in controls. Aims: (1) To assess the diversity of microbial communities of biopsy tissue using culture-independent methods; (2) to culture the bacteria found in the tissues of patients with IBD but not in the controls; (3) to identify potential virulence factors associated with cultured bacteria. Methods: 84 biopsy specimens were collected from 15 controls, 13 patients with Crohn’s disease (CD) and 19 patients with ulcerative colitis (UC) from a population-based case–control study. Ribosomal intergenic spacer analysis (RISA) was conducted to identify unique DNA bands in tissues from patients with CD and UC that did not appear in controls. Results: RISA followed by DNA sequencing identified unique bands in biopsy specimens from patients with IBD that were classified as Escherichia coli. Targeted culture showed a significantly (p<0.05) higher number of Enterobacteriaceae in specimens from patients with IBD. The B2+D phylogenetic group, serine protease autotransporters (SPATE) and adherence factors were more likely to be associated with tissues from patients with UC and CD than with controls. Conclusions: The abundance of Enterobacteriaceae is 3–4 logs higher in tissues of patients with IBD and the B2+D phylogenetic groups are more prevalent in patients with UC and CD. The B2+D phylogenetic groups are associated with SPATE and adherence factors and may have a significant role in disease aetiology.Keywords
This publication has 35 references indexed in Scilit:
- Gut-associated bacterial microbiota in paediatric patients with inflammatory bowel diseaseGut, 2006
- Enhanced persistence in the colonic microbiota of Escherichia coli strains belonging to phylogenetic group B2: role of virulence factors and adherence to colonic cellsMicrobes and Infection, 2006
- The Clustering of Other Chronic Inflammatory Diseases in Inflammatory Bowel Disease: A Population-Based StudyGastroenterology, 2005
- Secreted autotransporter toxin produced by a diffusely adheringEscherichia colistrain causes intestinal damage in animal model assaysFEMS Microbiology Letters, 2005
- Distribution of the Serine Protease Autotransporters of the Enterobacteriaceae among Extraintestinal Clinical Isolates of Escherichia coliJournal of Clinical Microbiology, 2005
- Risk factors for inflammatory bowel disease in the general populationAlimentary Pharmacology & Therapeutics, 2005
- Diversity of the Human Intestinal Microbial FloraScience, 2005
- Therapy Insight: vascular complications in patients with inflammatory bowel diseaseNature Clinical Practice Gastroenterology & Hepatology, 2005
- Molecular Characterization of Rectal Mucosa-Associated Bacterial Flora in Inflammatory Bowel DiseaseInflammatory Bowel Diseases, 2005
- Toward an Integrated Clinical, Molecular and Serological Classification of Inflammatory Bowel Disease: Report of a Working Party of the 2005 Montreal World Congress of GastroenterologyCanadian Journal of Gastroenterology, 2005