Simultaneous targeting of P‐gp and XIAP with siRNAs increases sensitivity of P‐gp overexpressing CML cells to imatinib
- 1 March 2011
- journal article
- research article
- Published by Taylor & Francis Ltd in Hematology
- Vol. 16 (2), 100-108
- https://doi.org/10.1179/102453311x12940641877803
Abstract
It is accepted that cancer chemoresistance may be due to overexpression of antiapoptotic proteins or P-gp. This study investigated the effect of downregulation of X-chromosome-linked inhibitor of apoptosis (XIAP) and of simultaneous downregulation of XIAP and P-gp on sensitivity to imatinib. The K562 and K562Dox (P-gp overexpressing) chronic myeloid leukemia cell lines were used and downregulation of target proteins was achieved with siRNAs. Targeting XIAP moderately enhanced sensitivity to imatinib in both cell lines. Simultaneous targeting of XIAP and P-gp further enhanced sensitivity to imatinib in the resistant K562Dox cells. In conclusion, simultaneous targeting of P-gp and XIAP increases sensitivity of P-gp overexpressing chronic myeloid leukemia cells to imatinib.Keywords
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