ADAMTS‐1 cleaves a cartilage proteoglycan, aggrecan
- 31 July 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 478 (3), 241-245
- https://doi.org/10.1016/s0014-5793(00)01854-8
Abstract
A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu(1871)-Leu(1872) bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo.Keywords
This publication has 31 references indexed in Scilit:
- Cloning and Characterization of ADAMTS11 , an Aggrecanase from the ADAMTS FamilyPublished by Elsevier BV ,1999
- Expression of ADAMTS Homologues in Articular CartilageBiochemical and Biophysical Research Communications, 1999
- Purification and Cloning of Aggrecanase-1: A Member of the ADAMTS Family of ProteinsScience, 1999
- Generation and Characterization of AggrecanasePublished by Elsevier BV ,1999
- The Exon/Intron Organization and Chromosomal Mapping of the Mouse ADAMTS-1 Gene Encoding an ADAM Family Protein with TSP MotifsGenomics, 1997
- Aggrecan degradation in human cartilage. Evidence for both matrix metalloproteinase and aggrecanase activity in normal, osteoarthritic, and rheumatoid joints.JCI Insight, 1997
- Molecular Cloning of a Gene Encoding a New Type of Metalloproteinase-disintegrin Family Protein with Thrombospondin Motifs as an Inflammation Associated GenePublished by Elsevier BV ,1997
- The ins and outs of aggrecanTrends in Cell Biology, 1995
- ADAM, a novel family of membrane proteins containing A Disintegrin And Metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions.The Journal of cell biology, 1995
- Cell-mediated Catabolism of AggrecanPublished by Elsevier BV ,1995