Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
- 17 June 1977
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4296), 1313-1319
- https://doi.org/10.1126/science.325648
Abstract
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.Keywords
This publication has 24 references indexed in Scilit:
- Chemical Synthesis of Restriction Enzyme Recognition Sites Useful for CloningScience, 1977
- The role of organelles in the chemical modification of the primary translation products of secretory proteinsFEBS Letters, 1976
- Sequence analysis of the 3′ non-coding regions of rabbit α- and β-globin messenger RNAsJournal of Molecular Biology, 1976
- The evolutionary origin of proinsulin: Amino acid sequence homology with the trypsin-related serine proteases detected and evaluated by new statistical methodsJournal of Molecular Biology, 1976
- Enzymatic in vitro synthesis of globin genesCell, 1976
- ‘Single-stranded’ DNA from φX174 and M13 is cleaved by certain restriction endonucleasesNature, 1975
- DNA Sequencing TechniquesAnnual Review of Biochemistry, 1974
- Ribonucleic acid isolated by cesium chloride centrifugationBiochemistry, 1974
- Rat-Proinsulin C-Peptides. Amino-Acid SequencesEuropean Journal of Biochemistry, 1972
- Species variation in the amino acid sequence of insulinAmerican Journal Of Medicine, 1966