Chemical Synthesis of Restriction Enzyme Recognition Sites Useful for Cloning
- 8 April 1977
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286), 177-180
- https://doi.org/10.1126/science.847463
Abstract
By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.Keywords
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