A bacterial clone synthesizing proinsulin.
- 1 August 1978
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 75 (8), 3727-3731
- https://doi.org/10.1073/pnas.75.8.3727
Abstract
We have cloned double-stranded cDNA copies of a rat preproinsulin messenger RNA in Escherichia coli chi1776, using the unique Pst endonuclease site of plasmid pBR322 that lies in the region encoding amino acids 181-182 of penicillinase. This site was reconstructed by inserting the cDNA with an oligo(dG)-oligo(dC) joining procedure. One of the clones expresses a fused protein bearing both insulin and penicillinase antigenic determinants. The DNA sequence of this plasmid shows that the insulin region is read in phase; a stretch of six glycine residues connects the alanine at position 182 of penicillinase to the fourth amino acid, glutamine, of rat proinsulin.Keywords
This publication has 33 references indexed in Scilit:
- The rabbit β-globin gene contains a large insert in the coding sequenceCell, 1977
- Ovalbumin gene is split in chicken DNANature, 1977
- Enzymatic in vitro synthesis of globin genesCell, 1976
- Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.The Journal of cell biology, 1975
- Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.The Journal of cell biology, 1975
- Genetic studies with heteroduplex DNA of bacteriophage f1. Asymmetric segregation, base correction and implications for the mechanism of genetic recombinationJournal of Molecular Biology, 1975
- Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acidBiochemistry, 1974
- Rat-Proinsulin C-Peptides. Amino-Acid SequencesJBIC Journal of Biological Inorganic Chemistry, 1972
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969
- Species variation in the amino acid sequence of insulinAmerican Journal Of Medicine, 1966