Palmitoylation regulates raft affinity for the majority of integral raft proteins
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- 3 December 2010
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (51), 22050-22054
- https://doi.org/10.1073/pnas.1016184107
Abstract
The physical basis for protein partitioning into lipid rafts remains an outstanding question in membrane biology that has previously been addressed only through indirect techniques involving differential solubilization by nonionic detergents. We have used giant plasma membrane vesicles, a plasma membrane model system that phase separates to include an ordered phase enriching for raft constituents, to measure the partitioning of the transmembrane linker for activation of T cells (LAT). LAT enrichment in the raft phase was dependent on palmitoylation at two juxtamembrane cysteines and could be enhanced by oligomerization. This palmitoylation requirement was also shown to regulate raft phase association for the majority of integral raft proteins. Because cysteine palmitoylation is the only lipid modification that has been shown to be reversibly regulated, our data suggest a role for palmitoylation as a dynamic raft targeting mechanism for transmembrane proteins.Keywords
This publication has 39 references indexed in Scilit:
- Temperature-dependent phase behavior and protein partitioning in giant plasma membrane vesiclesBiochimica et Biophysica Acta (BBA) - Biomembranes, 2010
- Hemagglutinin of Influenza Virus Partitions into the Nonraft Domain of Model MembranesBiophysical Journal, 2010
- Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesiclesBiochemical Journal, 2009
- Order of lipid phases in model and plasma membranesProceedings of the National Academy of Sciences of the United States of America, 2009
- Palmitoylation-Dependent Plasma Membrane Transport but Lipid Raft-Independent Signaling by Linker for Activation of T CellsThe Journal of Immunology, 2009
- Generation of Cubic Membranes by Controlled Homotypic Interaction of Membrane Proteins in the Endoplasmic ReticulumJournal of Biological Chemistry, 2009
- Have we become overly reliant on lipid rafts?EMBO Reports, 2008
- Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesiclesBiochimica et Biophysica Acta (BBA) - Biomembranes, 2008
- Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesiclesProceedings of the National Academy of Sciences of the United States of America, 2007
- Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live CellsScience, 2002