The Inhibitory Effect ofPrunella vulgarisL. on Aldose Reductase and Protein Glycation
Open Access
- 3 October 2012
- journal article
- research article
- Published by Hindawi Limited in Journal of Biomedicine and Biotechnology
- Vol. 2012, 1-7
- https://doi.org/10.1155/2012/928159
Abstract
To evaluate the aldose reductase (AR) enzyme inhibitory ability of Prunella vulgaris L. extract, six compounds were isolated and tested for their effects. The components were subjected to in vitro bioassays to investigate their inhibitory assays using rat lens aldose reductase (rAR) and human recombinant AR (rhAR). Among them, caffeic acid ethylene ester showed the potent inhibition, with the IC50 values of rAR and rhAR at 3.2±0.55μM and 12.58±0.32μM, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, this compound showed noncompetitive inhibition against rhAR. Furthermore, it inhibited galactitol formation in a rat lens incubated with a high concentration of galactose. Also it has antioxidative as well as advanced glycation end products (AGEs) inhibitory effects. As a result, this compound could be offered as a leading compound for further study as a new natural products drug for diabetic complications.Keywords
Funding Information
- National Research Foundation of Korea (2011-0030750, 2010-0001266, HRF-201112-002)
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