Vitamin D alleviates lipopolysaccharide-induced acute lung injury via regulation of the renin-angiotensin system
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Open Access
- 1 May 2017
- journal article
- Published by Spandidos Publications in Molecular Medicine Reports
- Vol. 16 (5), 7432-7438
- https://doi.org/10.3892/mmr.2017.7546
Abstract
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are the clinical manifestations of severe lung damage and respiratory failure. ALI and ARDS result are associated with high mortality in patients. At present, no effective treatments for ALI and ARDS exist. It is established that vitamin D exhibits anti‑inflammatory effects, however, the specific effect of vitamin D on ALI remains largely unknown. The aim of the present study was to investigate whether, and by which mechanism, vitamin D alleviates lipopolysaccharide (LPS)‑induced ALI. The results demonstrated that a vitamin D agonist, calcitriol, exhibited a beneficial effect on LPS‑induced ALI in rats; calcitriol pretreatment significantly improved LPS‑induced lung permeability, as determined using Evans blue dye. Results from reverse transcription‑quantitative polymerase chain reaction, western blotting and ELISA analysis demonstrated that calcitriol also modulated the expression of members of the renin‑angiotensin system (RAS), including angiotensin (Ang) I‑converting enzymes (ACE and ACE2), renin and Ang II, which indicates that calcitriol may exert protective effects on LPS‑induced lung injury, at least partially, by regulating the balance between the expression of members of the RAS. The results of the present study may provide novel targets for the future treatment of ALI.Keywords
This publication has 35 references indexed in Scilit:
- Angiotensin-converting enzyme 2, angiotensin-(1–7) and Mas: new players of the renin–angiotensin systemJournal of Endocrinology, 2012
- 1,25-Dihydroxyvitamin D3 and IL-2 Combine to Inhibit T Cell Production of Inflammatory Cytokines and Promote Development of Regulatory T Cells Expressing CTLA-4 and FoxP3The Journal of Immunology, 2009
- CD4+CD25+Foxp3+ Tregs resolve experimental lung injury in mice and are present in humans with acute lung injuryJCI Insight, 2009
- Has Mortality from Acute Respiratory Distress Syndrome Decreased over Time?American Journal of Respiratory and Critical Care Medicine, 2009
- Animal models of acute lung injuryAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2008
- Vitamin D: a negative endocrine regulator of the renin–angiotensin system and blood pressureThe Journal of Steroid Biochemistry and Molecular Biology, 2004
- 1,25-Dihydroxyvitamin D3 is a negative endocrine regulator of the renin-angiotensin systemJCI Insight, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- A Human Homolog of Angiotensin-converting EnzymeJournal of Biological Chemistry, 2000
- The Acute Respiratory Distress SyndromeThe New England Journal of Medicine, 2000