High-Throughput and High-Resolution Flow Cytometry in Molded Microfluidic Devices
- 13 July 2006
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (16), 5653-5663
- https://doi.org/10.1021/ac060340o
Abstract
We describe the design, fabrication, and operation of two types of flow cytometers based on microfluidic devices made of a single cast of poly(dimethylsiloxane). The stream of particles or cells injected into the devices is hydrodynamically focused in both transverse and lateral directions, has a uniform velocity, and has adjustable diameter and shape. The cytometry system built around the first microfluidic device has fluorescence detection accuracy comparable with that of a commercial flow cytometer and can analyze as many as 17 000 particles/s. This high-throughput microfluidic device could be used in inexpensive stand-alone cytometers or as a part of integrated microanalysis systems. In the second device, a stream of particles is focused to a flow layer of a submicrometer thickness that allows imaging the particles with a high numerical aperture microscope objective. To take long-exposure, low-light fluorescence images of live cells, the device is placed on a moving stage, which accurately balances the translational motion of particles in the flow. The achieved resolution is comparable to that of still micrographs. This high-resolution device could be used for analysis of morphology and fluorescence distribution in cells in continuous flow.Keywords
This publication has 15 references indexed in Scilit:
- Fluidic photonic integrated circuit for in-line detectionApplied Physics Letters, 2005
- An Axisymmetric Flow‐Focusing Microfluidic DeviceAdvanced Materials, 2005
- Three-Dimensional Hydrodynamic Focusing in Polydimethylsiloxane (PDMS) MicrochannelsJournal of Microelectromechanical Systems, 2004
- Plastic microchip flow cytometer based on 2- and 3-dimensional hydrodynamic flow focusingMicrosystem Technologies, 2003
- An Integrated Microfluidic System for Reaction, High-Sensitivity Detection, and Sorting of Fluorescent Cells and ParticlesAnalytical Chemistry, 2003
- Microfluidic Memory and Control DevicesScience, 2003
- Modular approach to fabrication of three-dimensional microchannel systems in PDMS—application to sheath flow microchipsLab on a Chip, 2001
- Flow Cytometry of Escherichia coli on Microfluidic DevicesAnalytical Chemistry, 2001
- Lifetimes of intermediates in the β-sheet to α-helix transition of β-lactoglobulin by using a diffusional IR mixerProceedings of the National Academy of Sciences, 2001
- Microchip Flow Cytometry Using Electrokinetic FocusingAnalytical Chemistry, 1999