An In Vitro Study of Osteoblast Response on Fused-Filament Fabrication 3D Printed PEEK for Dental and Cranio-Maxillofacial Implants
Open Access
- 31 May 2019
- journal article
- research article
- Published by MDPI AG in Journal of Clinical Medicine
- Vol. 8 (6), 771
- https://doi.org/10.3390/jcm8060771
Abstract
Polyetheretherketone (PEEK) is a prime candidate to replace metallic implants and prostheses in orthopedic, spine and cranio-maxillofacial surgeries. Fused-filament fabrication (FFF) is an economical and efficient three-dimensional (3D) printing method to fabricate PEEK implants. However, studies pertaining to the bioactivity of FFF 3D printed PEEK are still lacking. In this study, FFF 3D printed PEEK samples were fabricated and modified with polishing and grit-blasting (three alumina sizes: 50, 120, and 250 µm) to achieve varying levels of surface roughness. In vitro cellular response of a human osteosarcoma cell line (SAOS-2 osteoblasts, cell adhesion, metabolic activity, and proliferation) on different sample surfaces of untreated, polished, and grit-blasted PEEK were evaluated. The results revealed that the initial cell adhesion on different sample surfaces was similar. However, after 5 days the untreated FFF 3D printed PEEK surfaces exhibited a significant increase in cell metabolic activity and proliferation with a higher density of osteoblasts compared with the polished and grit-blasted groups (p < 0.05). Therefore, untreated FFF 3D printed PEEK with high surface roughness and optimal printing structures might have great potential as an appropriate alloplastic biomaterial for reconstructive cranio-maxillofacial surgeries.Keywords
Funding Information
- China Scholarship Council (201606280045, 201708080003)
This publication has 37 references indexed in Scilit:
- Cytocompatibility, osseointegration, and bioactivity of three-dimensional porous and nanostructured network on polyetheretherketoneBiomaterials, 2013
- Role of trapped air in the formation of cell-and-protein micropatterns on superhydrophobic/superhydrophilic microtemplated surfacesBiomaterials, 2012
- Cranioplasty with Customized Titanium and PEEK Implants in a Mechanical Stress ModelJournal of Neurotrauma, 2012
- Stress shielding and fatigue limits of poly‐ether‐ether‐ketone dental implantsJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2012
- The future of dental devices is digitalDental Materials, 2012
- Wettability versus roughness of engineering surfacesWear, 2011
- Early osseointegration to hydrophilic and hydrophobic implant surfaces in humansClinical Oral Implants Research, 2011
- Effect of roughness, wettability and morphology of engineered titanium surfaces on osteoblast-like cell adhesionColloids and Surfaces A: Physicochemical and Engineering Aspects, 2010
- Tuning cell adhesion by controlling the roughness and wettability of 3D micro/nano silicon structuresActa Biomaterialia, 2010
- The influence of sterilization processes on the micromechanical properties of carbon fiber-reinforced PEEK composites for bone implant applicationsActa Biomaterialia, 2007