Improvement of the skin flap survival with the bone marrow‐derived mononuclear cells transplantation in a rat model
- 22 March 2010
- journal article
- research article
- Published by Wiley in Microsurgery
- Vol. 30 (4), 275-281
- https://doi.org/10.1002/micr.20779
Abstract
Partial necrosis of skin flaps remains a significant problem in plastic and reconstructive surgery. In this study we attempted to evaluate the effect of bone marrow‐derived mononuclear cells (BM‐MNCs) transplantation on improvement of skin flap survival in a rat random pattern skin flap model. Thirty Wistar rats were divided into three groups with each consisting of 10 rats. BM‐MNCs and the adipose‐derived stem cells (ADSCs) were transplanted into the subcutaneous tissue in the area where the flap would be dissected. The flaps were then raised two days after cells transplantation. The animals receiving the preoperative Dulbecco's Modified Eagle Medium (DMEM) treatment were used as the controls. On the 7th postoperative day, the survival areas of flaps were measured and tissues were collected for examinations. The results showed that the mean survival areas were 46.33 ± 13.46% in the ADSCs group and 50.06 ± 13.82% in the BM‐MNCs group as the percentages of the total skin flaps, which were significantly higher than that in the control group (26.33 ± 7.14%) (P < 0.05). Histological analysis showed increased neovascularization in the flap treated with BM‐MNCs when compared with ADSCs transplantation. Survival BM‐MNCs and ADSCs were detected in the flap tissues. Higher levels of the basic fibroblast growth factor (bFGF) and vascular endothelium growth factor (VEGF) were found in the BM‐MNCs transplantation group (P < 0.05). The findings from this study demonstrated that preoperative treatment with BM‐MNCs transplantation could promote neovascularization and improve flap survival. These effects of BM‐MNCs on flap survival were comparable with ADSCs transplantation, but without necessity of in vitro cells expansion. © 2010 Wiley‐Liss, Inc. Microsurgery, 2010Keywords
Funding Information
- Key Project of National Natural Science Foundation (30730092)
This publication has 32 references indexed in Scilit:
- Adipose tissue: a new source for cardiovascular repairJournal of Cardiovascular Medicine, 2010
- Novel Cell-Based Therapeutic Strategy for Ischemic Colitis with Use of Bone Marrow–Derived Mononuclear Cells in RatsDiseases of the Colon & Rectum, 2009
- Cardiovascular cell therapy and endogenous repairDiabetes, Obesity and Metabolism, 2008
- Transvenous Intramyocardial Cellular Delivery Increases Retention in Comparison to Intracoronary Delivery in a Porcine Model of Acute Myocardial InfarctionJournal of Interventional Cardiology, 2008
- Changes in angiogenesis-related factors in serum following autologous bone marrow cell implantation for severe limb ischemiaExpert Opinion on Biological Therapy, 2008
- Gene therapy with adenovirus-mediated VEGF enhances skin flap prefabricationMicrosurgery, 2005
- Therapeutic stem and progenitor cell transplantation for organ vascularization and regenerationNature Medicine, 2003
- Effects of VEGF Administration Following Ischemia on Survival of the Gracilis Muscle Flap in the RatAnnals of Plastic Surgery, 1999
- Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularizationJCI Insight, 1999
- Evidence for the Efficacy of TRAM Flap Delay in a Rat ModelPlastic and Reconstructive Surgery, 1995