Novel Cell-Based Therapeutic Strategy for Ischemic Colitis with Use of Bone Marrow–Derived Mononuclear Cells in Rats
- 1 August 2009
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Diseases of the Colon & Rectum
- Vol. 52 (8), 1443-1451
- https://doi.org/10.1007/dcr.0b013e3181a79549
Abstract
Ischemic colitis is a common disorder of the large bowel. In the clinical setting, some patients suffer refractory ischemic colitis regardless of conventional treatment. Meanwhile, bone marrow–derived mononuclear cells are known to accelerate neovascularization. The purpose of this study was to verify the effects of bone marrow–derived mononuclear cells on ischemic colitis in rats. An ischemic colitis model was established by partial obstruction of the rectum and interruption of the marginal vessel in the immunodeficient rat. Bone marrow–derived mononuclear cells from a Wistar rat were injected into the ischemic area one day later than the ischemia (Group MNC). As a control, phosphate-buffered saline was injected in the same manner (Group PBS). Seven days after cell transplantation, each rat was evaluated for histology and colic motility. Compared with Group PBS scores, the Group MNC macroscopic and microscopic colitis severity scores were significantly reduced. Moreover, the density of the capillary and myenteric plexus was significantly higher in Group MNC than in Group PBS (9.55 ± 0.74 vs. 4.61 ± 0.22, respectively, P CONCLUSIONS: These results suggest that transplantation of bone marrow–derived mononuclear cells might enhance not only tissue regeneration and angiogenesis but also neurogenesis. Transplantation of bone marrow–derived mononuclear cells may be a useful therapeutic strategy for ischemic colitis.Keywords
This publication has 37 references indexed in Scilit:
- The Effect of Sildenafil on an Animal Model for Ischemic ColitisDigestive Diseases and Sciences, 2007
- Nonmyeloablative Stem Cell Therapy Enhances Microcirculation and Tissue Regeneration in Murine Inflammatory Bowel DiseaseGastroenterology, 2007
- Axonal sprouting into the denervated spinal cord and synaptic and postsynaptic protein expression in the spinal cord after transplantation of bone marrow stromal cell in stroke ratsBrain Research, 2007
- Additive effect of endothelial progenitor cell mobilization and bone marrow mononuclear cell transplantation on angiogenesis in mouse ischemic limbsJournal of Biomedical Science, 2007
- Bone marrow transplantation ameliorates pathology in interleukin-10 knockout colitic miceThe Journal of Pathology, 2006
- Chronic ischaemic bowel diseases in the aged—go with the flowAge and Ageing, 2005
- Endothelial Progenitor CellsCirculation Research, 2004
- Therapeutic stem and progenitor cell transplantation for organ vascularization and regenerationNature Medicine, 2003
- Effector mechanisms in murine allograft rejection: comparison of skin and heart grafts in fully allogeneic and minor histocompatibility antigen-mismatched strain combinationsTransplant International, 2002
- Age-related changes in the colonic blood supply: their relevance to ischaemic colitis.Gut, 1978