Aberration compensation in confocal microscopy
- 1 September 1991
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 30 (25), 3563-3568
- https://doi.org/10.1364/ao.30.003563
Abstract
In confocal microscopy, spherical aberration is introduced when one is focusing deep within the specimen. This can be compensated for by altering the effective tube length at which the objective is operated. The limitations of this approach are investigated.Keywords
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