Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu+RSV Xpress Assay in Point-of-Care Settings: Comparison to Prodesse ProFlu+
Open Access
- 1 February 2018
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 56 (2), e01237-17
- https://doi.org/10.1128/jcm.01237-17
Abstract
The Xpert Flu+RSV Xpress Assay is a fast, automated in vitro diagnostic test for qualitative detection and differentiation of influenza A and B viruses and respiratory syncytial virus (RSV) performed on the Cepheid GeneXpert Xpress System. The objective of this study was to establish performance characteristics of the Xpert Flu+RSV Xpress Assay compared to those of the Prodesse ProFlu+ real-time reverse transcription-PCR (RT-PCR) assay (ProFlu+) for the detection of influenza A and B viruses as well as RSV in a Clinical Laboratory Improvement Amendments (CLIA)-waived (CW) setting. Overall, the assay, using fresh and frozen nasopharyngeal (NP) swabs, demonstrated high concordance with results of the ProFlu+ assay in the combined CW and non-CW settings with positive percent agreements (PPA) (100%, 100%, and 97.1%) and negative percent agreements (NPA) (95.2%, 99.5%, and 99.6%) for influenza A and B viruses and RSV, respectively. In conclusion, this multicenter study using the Cepheid Xpert Flu+RSV Xpress Assay demonstrated high sensitivities and specificities for influenza A and B viruses and RSV in ∼60 min for use at the point-of-care in the CW setting.Keywords
Funding Information
- Cepheid, Inc.
This publication has 18 references indexed in Scilit:
- Evaluation of the Alere i Influenza A&B assay for rapid identification of influenza A and influenza B virusesJournal of Medical Microbiology, 2016
- Prospective evaluation of the Alere i Influenza A&B nucleic acid amplification versus Xpert Flu/RSVDiagnostic Microbiology and Infectious Disease, 2016
- Utility of early influenza diagnosis through point‐of‐care testing in children presenting to an emergency departmentJournal of Paediatrics and Child Health, 2016
- Economic Impact of a New Rapid PCR Assay for Detecting Influenza Virus in an Emergency Department and Hospitalized PatientsPLOS ONE, 2016
- Analytical performance of the Alere™ i Influenza A&B assay for the rapid detection of influenza virusesEnfermedades Infecciosas y Microbiología Clínica, 2015
- Point-of-care testing for respiratory viruses in adults: The current landscape and future potentialJournal of Infection, 2015
- Performance of the Molecular Alere i Influenza A&B Test Compared to That of the Xpert Flu A/B AssayJournal of Clinical Microbiology, 2015
- Multicenter clinical evaluation of the novel Alere™ i Influenza A&B isothermal nucleic acid amplification testJournal of Clinical Virology, 2014
- Predictors of severe H1N1 infection in children presenting within Pediatric Emergency Research Networks (PERN): retrospective case-control studyBMJ, 2013
- InfluenzaMedical Clinics of North America, 2013