Blood Leukocyte mRNA Expression for IL-10, IL-1Ra, and IL-8, but Not IL-6, Increases After Exercise
- 1 September 2006
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon & Cytokine Research
- Vol. 26 (9), 668-674
- https://doi.org/10.1089/jir.2006.26.668
Abstract
The primary purpose of this project was to study exercise-induced leukocyte cytokine mRNA expression. Changes in plasma cytokine levels and blood leukocyte mRNA expression for interleukin-6 (IL-6), IL-8, IL- 10, and IL-1 receptor antagonist (IL-1Ra) were measured in 12 athletes following 2 h of intensive cycling (∼64% Wattsmax) while ingesting a carbohydrate or placebo beverage (randomized and double blinded). Blood samples were collected 30 min preexercise and immediately and 1 h postexercise. Carbohydate compared with placebo ingestion attenuated exercise-induced changes in plasma cortisol (8.8% vs. 62%, respectively), epinephrine (–9.2% vs. 138%), IL-6 (10-fold vs. 40-fold), IL-10 (8.9-fold vs. 26-fold, and IL-1Ra (2.1-fold vs. 5.6-fold). Significant time effects were measured for blood leukocyte IL-8 (2.4-fold increase 1 h postexercise), IL-10 (2.7-fold increase), IL-1Ra (2.2-fold increase), and IL-6 (0.8-fold decrease) mRNA content, with no significant differences between Cho and Pla test conditions. In summary, gene expression for IL-8, IL-10, and IL-1Ra, but not IL-6, is increased in blood leukocytes taken from athletes following 2 h of intensive cycling and is not influenced by carbohydrate compared with placebo ingestion. mRNA expression was high enough to indicate a substantial contribution of blood leukocytes to plasma levels of IL-8, IL-10, and IL-1Ra during prolonged exercise.Keywords
This publication has 27 references indexed in Scilit:
- Muscle damage is linked to cytokine changes following a 160-km raceBrain, Behavior, and Immunity, 2005
- Muscle Cytokine mRNA Changes after 2.5 h of Cycling: Influence of CarbohydrateMedicine & Science in Sports & Exercise, 2005
- Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availabilityAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2004
- Influence of carbohydrate ingestion on immune changes after 2 h of intensive resistance trainingJournal of Applied Physiology, 2004
- Immunohistochemical detection of interleukin‐6 in human skeletal muscle fibers following exerciseThe FASEB Journal, 2003
- Carbohydrate ingestion influences skeletal muscle cytokine mRNA and plasma cytokine levels after a 3-h runJournal of Applied Physiology, 2003
- Cytokine changes after a marathon raceJournal of Applied Physiology, 2001
- Influence of mode and carbohydrate on the cytokine response to heavy exertionMedicine & Science in Sports & Exercise, 1998
- Evidence that interleukin‐6 is produced in human skeletal muscle during prolonged runningJournal Of Physiology-London, 1998
- Carbohydrate and the cytokine response to 2.5 h of runningJournal of Applied Physiology, 1997