Liposomes as Protective Capsules for Active Silica Sol−Gel Biocomposite Synthesis
- 24 August 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (37), 12756-12757
- https://doi.org/10.1021/ja0525354
Abstract
Using liposome to shield an enzyme from hostile chemical environments during the sol−gel formation process has resulted in a novel approach to synthesizing silica sol−gel biocomposite materials. By reporting the encapsulation of horseradish peroxidase and firefly luciferase, we demonstrate that this new protocol can produce silica biocomposites that are more active than trapping the enzymes directly into hydrogels.This publication has 13 references indexed in Scilit:
- Mechanism of horseradish peroxidase inactivation by benzhydrazide: a critical evaluation of arylhydrazides as peroxidase inhibitorsBiochemical Journal, 2003
- Using Sugar and Amino Acid Additives to Stabilize Enzymes within Sol−Gel Derived SilicaChemistry of Materials, 2003
- Controlling the Material Properties and Biological Activity of Lipase within Sol−Gel Derived Bioglasses via Organosilane and Polymer DopingChemistry of Materials, 2000
- Protecting Heme Enzyme Peroxidase Activity from H2O2 Inactivation by Sol−Gel EncapsulationLangmuir, 2000
- Aqueous Sol−Gel Process for Protein EncapsulationChemistry of Materials, 2000
- Using Intrinsic Fluorescence to Investigate Proteins Entrapped in Sol-Gel Derived MaterialsApplied Spectroscopy, 1999
- Encapsulation of Biologicals within Silicate, Siloxane, and Hybrid Sol−Gel Polymers: An Efficient and Generic ApproachJournal of the American Chemical Society, 1998
- Low Catalytic Turnover of Horseradish Peroxidase in Thiocyanate OxidationOnline Journal of Public Health Informatics, 1997
- Encapsulation of Proteins in Transparent Porous Silicate Glasses Prepared by the Sol-Gel MethodScience, 1992
- Biochemically active sol-gel glasses: the trapping of enzymesMaterials Letters, 1990