Simultaneous Determination of the Size and Surface Charge of Individual Nanoparticles Using a Carbon Nanotube-Based Coulter Counter

Abstract

A resistive-pulse Coulter counter based on a membrane containing a single multiwall carbon nanotube (MWNT) channel was used to simultaneously determine the size and surface charge of carboxy-terminated polystyrene nanoparticles. The membrane was prepared from an epoxy section containing a MWNT channel mounted on a poly(dimethylsiloxane) (PDMS) support structure. The PDMS support reduced the background noise level by a factor of >20 compared to the Si/Si₃N₄ support structure used in our previous study. The lower noise level makes it possible to accurately measure the height and width of resistive-pulse signals resulting from transport of individual particles through the MWNT channel. Particle sizes, calculated from current pulse heights, were comparable to those determined by transmission electron microscope (TEM). The width of the current pulses is a measure of the nanoparticle transport time, and it permits calculation of the electrokinetic surface charge. Different types of polystyrene nanoparticles having nearly the same size, but different electrokinetic surface charge, could be resolved on the basis of the difference in their transport time.