Involvement of miR-21 in resistance to daunorubicin by regulating PTEN expression in the leukaemia K562 cell line
- 25 December 2010
- journal article
- Published by Wiley in FEBS Letters
- Vol. 585 (2), 402-408
- https://doi.org/10.1016/j.febslet.2010.12.027
Abstract
Recent studies have shown microRNA-21 (miR-21) is overexpressed in several types of cancer and contributes to tumor resistance to chemotherapy. In this study, we investigated whether miR-21 mediated resistance of the leukaemia cell line K562 to the chemotherapeutic agent daunorubicin (DNR). miR-21 expression was upregulated in the DNR resistant cell line K562/DNR compared to its parental line K562. Stable transfection of miR-21 induced drug resistance in K562, while suppression of miR-21 in K562/DNR led to enhanced DNR cytotoxicity. Additional experiments indicate that the mechanism of miR-21 drug resistance involves the PI3K/Akt pathway and changes following PTEN protein expression. This study provides a novel mechanism for understanding leukaemia drug resistanceFunding Information
- National Natural Science Foundation of China (81070135)
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