Amino acid sequence of the Pronase-released heads of neuraminidase subtype N2 from the Asian strain A/Tokyo/3/67 of influenza virus

Abstract

The amino acid sequence of the pronase-released heads of neuraminidase subtype N2 from the A/Tokyo/3/67 strain of influenza virus was determined by a combination of peptide and nucleic acid sequence analysis. The pronase-released heads contain 396 amino acid residues and extend from residue 74 in the original protein to the C-terminus at residue 469. The heads contain 5 potential glycosylation sites at asparagine residues 86, 146, 200, 234 and 402, but only the first 4 are glycosylated. The sequence homology with the corresponding region of the previously published sequence of the neuraminidase subtype N1 is 45%.