High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy
- 4 March 2007
- journal article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 4 (4), 311-313
- https://doi.org/10.1038/nmeth1017
Abstract
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.Keywords
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