Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas
Open Access
- 29 April 2020
- journal article
- research article
- Published by American Society for Microbiology in mSphere
- Vol. 5 (2)
- https://doi.org/10.1128/msphere.00310-20
Abstract
Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality. Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces. C. neoformans is encased by polysaccharide capsule that is highly hydrophilic and is a critical determinant of virulence. In this study, we report large differences in the CSH of some C. neoformans and C. gattii strains. The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed. We found that cell wall composition, in the context of chitin-chitosan content, does not influence CSH. For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii. Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains. Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains. IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell. The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans. The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba. Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.Keywords
Funding Information
- Irish Research Council (Postgraduate Research Award GOIPG/2016/998)
- HHS | NIH | National Heart, Lung, and Blood Institute (HL059842, AI052733, AI152078)
This publication has 98 references indexed in Scilit:
- Antibody Binding to Cryptococcus neoformans Impairs Budding by Altering Capsular Mechanical PropertiesThe Journal of Immunology, 2013
- Chronological Aging Is Associated with Biophysical and Chemical Changes in the Capsule of Cryptococcus neoformansInfection and Immunity, 2011
- Cell Wall Chitosan Is Necessary for Virulence in the Opportunistic Pathogen Cryptococcus neoformansEukaryotic Cell, 2011
- Evidence for branching in cryptococcal capsular polysaccharides and consequences on its biological activityMolecular Microbiology, 2011
- Lipophilic Dye Staining ofCryptococcus neoformansExtracellular Vesicles and CapsuleEukaryotic Cell, 2009
- Variation of cell surface hydrophobicity and biofilm formation among genotypes ofCandida albicansandCandida dubliniensisunder antifungal treatmentCanadian Journal of Microbiology, 2008
- Cryptococcus neoformans Biofilm Formation Depends on Surface Support and Carbon Source and Reduces Fungal Cell Susceptibility to Heat, Cold, and UV LightApplied and Environmental Microbiology, 2007
- Chitosan, the Deacetylated Form of Chitin, Is Necessary for Cell Wall Integrity in Cryptococcus neoformansEukaryotic Cell, 2007
- Dependence of Macrophage Phagocytic Efficacy on Antibody ConcentrationInfection and Immunity, 2007
- Characterization of a flocculation-like phenotype in Cryptococcus neoformans and its effects on pathogenesisCellular Microbiology, 2006