Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)
- 29 September 2020
- journal article
- research article
- Published by University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca in Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology
- Vol. 77 (2), 67-76
- https://doi.org/10.15835/buasvmcn-fst:2020.0041
Abstract
Copper metal as a contact surface was studied during the germination of alfalfa seeds (Medicago sativa L.) inside a rotating drum on a laboratory scale and compared with a plastic surface of food-grade. A system of three rotating drums was used inside a thermo-regulated chamber to germinate seeds. Alfalfa seeds were inoculated with 4.2 log cfu g-1 of Escherichia coli and after 84 h of germination sprouts were evaluated for E. coli, mesophilic aerobic bacteria, the content of copper and other minerals (potassium, calcium, magnesium, sodium, iron, manganese, and zinc), total mass, unit mass and length, and color. The contact of alfalfa sprouts with copper sheets allowed to reduce the E. coli load from 6.54 to 10 ppm) according to Food Sanitary Regulations. Germinated mass and length decreased after copper treatments. No statistically significant differences were observed between treatments for the remaining quality parameters. Finally, it is concluded that copper was very efficient in reducing the microbial load of E. coli in alfalfa sprouts, complying with the regulations established by the Chilean Ministry of Health.Keywords
This publication has 22 references indexed in Scilit:
- Mechanism of copper surface toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarization followed by slower rate of DNA destruction which differs from that observed for Gram‐positive bacteriaEnvironmental Microbiology, 2011
- Metallic Copper as an Antimicrobial SurfaceApplied and Environmental Microbiology, 2011
- Bacterial Killing by Dry Metallic Copper SurfacesApplied and Environmental Microbiology, 2011
- Efficacy of combinations of high pressure treatment, temperature and antimicrobial compounds to improve the microbiological quality of alfalfa seeds for sprout productionFood Control, 2009
- Potential application of high hydrostatic pressure to eliminate Escherichia coli O157:H7 on alfalfa sprouted seedsInternational Journal of Food Microbiology, 2008
- Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishmentsInternational Journal of Food Microbiology, 2008
- Contribution of Copper Ion Resistance to Survival of Escherichia coli on Metallic Copper SurfacesApplied and Environmental Microbiology, 2008
- Survival of Listeria monocytogenes Scott A on metal surfaces: Implications for cross-contaminationInternational Journal of Food Microbiology, 2006
- Use of Copper Cast Alloys To Control Escherichia coli O157 Cross-Contamination during Food ProcessingApplied and Environmental Microbiology, 2006
- The survival of Escherichia coli O157 on a range of metal surfacesInternational Journal of Food Microbiology, 2005