TRPC1 participates in the HSV-1 infection process by facilitating viral entry
Open Access
- 20 March 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 6 (12), eaaz3367
- https://doi.org/10.1126/sciadv.aaz3367
Abstract
Mammalian transient receptor potential (TRP) channels are major components of Ca2+ signaling pathways and control a diversity of physiological functions. Here, we report a specific role for TRPC1 in the entry of herpes simplex virus type 1 (HSV-1) into cells. HSV-1–induced Ca2+ release and entry were dependent on Orai1, STIM1, and TRPC1. Inhibition of Ca2+ entry or knockdown of these proteins attenuated viral entry and infection. HSV-1 glycoprotein D interacted with the third ectodomain of TRPC1, and this interaction facilitated viral entry. Knockout of TRPC1 attenuated HSV-1–induced ocular abnormality and morbidity in vivo in TRPC1−/− mice. There was a strong correlation between HSV-1 infection and plasma membrane localization of TRPC1 in epithelial cells within oral lesions in buccal biopsies from HSV-1–infected patients. Together, our findings demonstrate a critical role for TRPC1 in HSV-1 infection and suggest the channel as a potential target for anti-HSV therapy.Keywords
Funding Information
- National Natural Science Foundation of China (81622007)
- National Natural Science Foundation of China (81870362)
- Fundamental Research Funds for the Central Universities (JUSRP51704A)
- Chang Jiang Scholars Program (Q2015106)
- Intramural Research Program of the NIH (Z01-ES-101684)
- NIDCR-DIR, NIH (Z01-DE00438-31)
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