TrpC1 Is a Membrane-Spanning Subunit of Store-Operated Ca 2+ Channels in Native Vascular Smooth Muscle Cells
- 19 January 2001
- journal article
- other
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation Research
- Vol. 88 (1), 84-87
- https://doi.org/10.1161/01.res.88.1.84
Abstract
—Mammalian counterparts of the Drosophila trp gene have been suggested to encode store-operated Ca2+ channels. These specialized channels are widely distributed and may have a general function to reload Ca2+ into sarcoplasmic reticulum as well as specific functions, including the control of cell proliferation and muscle contraction. Heterologous expression of mammalian trp genes enhances or generates Ca2+ channel activity, but the crucial question of whether any of the genes encode native subunits of store-operated channels remains unanswered. We have investigated if TrpC1 protein (encoded by trp1 gene) is a store-operated channel in freshly isolated smooth muscle cells of resistance arterioles, arteries, and veins from human, mouse, or rabbit. Messenger RNA encoding TrpC1 was broadly expressed. TrpC1-specific antibody targeted to peptide predicted to contribute to the outer vestibule of TrpC1 channels revealed that TrpC1 is localized to the plasma membrane and has an extracellular domain. Peptide-specific binding of the antibody had a functional effect, selectively blocking store-operated Ca2+ channel activity. The antibody is a powerful new tool for the study of mammalian trp1 gene product. The study shows that TrpC1 is a novel physiological Ca2+ channel subunit in arterial smooth muscle cells.Keywords
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