Surface plasmon resonance sensing of Ebola virus: a biological threat
- 19 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 412 (17), 4101-4112
- https://doi.org/10.1007/s00216-020-02641-5
Abstract
Here, different monoclonal antibodies (mAb1, mAb2 and mAb3) of Ebola virus were screened in a real-time and label-free manner using surface plasmon resonance (SPR) to select an appropriate antibody for biosensor applications against a biological warfare agent. For this purpose, a gold SPR chip was modified with 4-mercaptobenzoic acid (4-MBA), and modification was confirmed by FTIR-ATR and EIS. The 4-MBA-modified gold SPR chip was used for immobilization of the recombinant nucleoprotein of Ebola (EBOV-rNP), and the interactions of mAb1, mAb2 and mAb3 were then investigated to determine the best mAb based on the affinity constant (KD), expressed as equilibrium dissociation constant. KD values of 809 nM, 350 pM and 52 pM were found for the interaction of mAb1, mAb2 and mAb3 of Ebola with the immobilized EBOV-rNP, respectively, thus reflecting the high affinity of mAb3. This was confirmed by ELISA results. The thermodynamic parameters (ΔG, ΔH and ΔS) for the interaction between mAb3 and EBOV-rNP were also determined, which revealed that the interaction was spontaneous, endothermic and driven by entropy. The SPR limit of detection of EBOV-rNP with mAb3 was 0.5 pg ml−1, showing mAb3 to be the best high-affinity antibody in our study. This study has opened up new possibilities for SPR screening of different monoclonal antibodies of BWA through the convergence of materials science and optical techniques.Keywords
This publication has 50 references indexed in Scilit:
- Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon ElectrodeSensors, 2013
- High Affinity Antigen Recognition of the Dual Specific Variants of Herceptin Is Entropy-Driven in Spite of Structural PlasticityPLOS ONE, 2011
- Surface-Enhanced Raman Spectroscopy Investigation of the Potential-Dependent Acid−Base Chemistry of Silver-Immobilized 2-Mercaptobenzoic AcidLangmuir, 2011
- Supersensitive detection of T-2 toxin by the in situ synthesized π-conjugated molecularly imprinted nanopatterns. An in situ investigation by surface plasmon resonance combined with electrochemistryBiosensors and Bioelectronics, 2011
- Biomolecular Interaction Study of Cyclolinopeptide A with Human Serum AlbuminJournal of Biomedicine and Biotechnology, 2010
- Impedance Biosensor for Peanut Protein Ara h 1Analytical Chemistry, 2008
- Electrochemical and infrared spectroscopy studies of 4-mercaptobenzoic acid SAMs on gold surfacesElectrochimica Acta, 2008
- Hydrophobic, hydrophilic and other interactions in epitope-paratope bindingMolecular Immunology, 1995
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970